Precipitación de carbonatos inducida microbiológicamente como potencial estrategia en la restauración de estructuras patrimoniales
DOI:
https://doi.org/10.37558/gec.v21i1.1119Palavras-chave:
precipitación de carbonatos inducida microbiológicamente, restauración, conservación, patrimonio cultural, calcitaResumo
En los últimos años, la precipitación de carbonatos inducida microbiológicamente ha demostrado ser una potencial herramienta de restauración de piezas arquitectónicas y esculturas alrededor del mundo con buenos resultados a corto y largo plazo. En este trabajo se realizaron incubaciones partiendo de cepas bacterianas locales de Bacillus subtilis previamente aisladas e identificadas para comprobar la capacidad de estas cepas bacterianas en la producción de carbonato de calcio sobre muestras de roca carbonática. Los cristales obtenidos fueron analizados a través de FT-IR, SEM-EDS y DRX. Los resultados muestran la formación de películas estables de bioprecipitado de calcita, el polimorfo más estable de carbonato de calcio, sobre las muestras de rocas demostrando así la posibilidad de utilizar cepas no patogénicas y medios económicos para restaurar, prevenir o disminuir el futuro deterioro del patrimonio cultural.
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Referências
AL-THAWADI, S. M. (2011). ‘Ureolytic bacteria and calcium carbonate formation as a mechanism of strength enhancement of sand’, Journal of Advanced Science and Engineering Research, 1. Available at: https://www.sign-ific-ance.co.uk/dsr/index.php/JASER/article/view/26.
ALLEN, T. (no date) Serial Dilution Problem # 1. Available at: https://www.uvm.edu/~btessman/calc/serhelp.html.
ANDREOLLI, M. et al. (2020). ‘Bacteria from black crusts on stone monuments can precipitate CaCO3 allowing the development of a new bio-consolidation protocol for ornamental stone’, International Biodeterioration & Biodegradation, 153. https://doi.org/10.1016/j.ibiod.2020.105031. DOI: https://doi.org/10.1016/j.ibiod.2020.105031
AWAIS, M. et al. (2007). ‘Isolation, identification and optimization of bacitracin produced by Bacillus SP.’, Pakistan Journal of Botany, 39(4): 1303–1312.
BANG, S. S., GALINAT, J. K. AND RAMAKRISHNAN, V. (2001). ‘Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii’, Enzyme and Microbial Technology, 28(4–5): 404–409. https://doi.org/10.1016/S0141-0229(00)00348-3. DOI: https://doi.org/10.1016/S0141-0229(00)00348-3
CAMAITI, M., BORSELLI, G. AND MATTEOLI, U. (1988). La conservazione del patrimonio monumentale: Prodotti consolidanti impiegati nelle operazioni de restauro. 10th edn. Edited by s.e. s.l.: L’edilizia e l’ industrializazzione.
CARRIÓ, V. AND MARCOS, F. (2013). ‘Pros and Cons of Restoration’, 22nd Symposium for Palaeontological Preparation and Conservation Geological Curators’ Group, (November), 12. https://doi.org/10.13140/RG.2.2.19704.75526.
CHAPARRO-ACUÑA, S. P. et al. (2018) ‘Soil bacteria that precipitate calcium carbonate: Mechanism and applications of the process’, Acta Agronomica, 67(2). https://doi.org/10.15446/acag.v67n2.66109. DOI: https://doi.org/10.15446/acag.v67n2.66109
CHOI, S.-G. et al. (2020) ‘Review on geotechnical engineering properties of sands treated by microbially induced calcium carbonate precipitation (MICP) and biopolymers’, Construction and Building Materials, 246(118415). https://doi.org/10.1016/j.conbuildmat.2020.118415. DOI: https://doi.org/10.1016/j.conbuildmat.2020.118415
DASKALAKIS, M. I. et al. (2013) ‘Pseudomonas , Pantoea and Cupriavidus isolates induce calcium carbonate precipitation for biorestoration of ornamental stone’, Journal of Applied Microbiology, 115(2): 409–423. https://doi.org/10.1111/jam.12234. DOI: https://doi.org/10.1111/jam.12234
DICK, J. et al. (2006) ‘Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species’, Biodegradation, 17(4): 357–367. https://doi.org/10.1007/s10532-005-9006-x. DOI: https://doi.org/10.1007/s10532-005-9006-x
GARCÍA-GONZÁLEZ, J. et al. (2017) ‘Quality improvement of mixed and ceramic recycled aggregates by biodeposition of calcium carbonate’, Construction and Building Materials, 154: 1015–1023. https://doi.org/10.1016/j.conbuildmat.2017.08.039. DOI: https://doi.org/10.1016/j.conbuildmat.2017.08.039
GIORGI, R. et al. (2010) ‘New Methodologies for the conservation of cultural heritage: Micellar solutions, microemulsions, and hydroxide nanoparticles’, Accounts of Chemical Research, 43(6): 2. https://doi.org/10.1021/ar900193h. DOI: https://doi.org/10.1021/ar900193h
Jokilehto, J. (2005) ‘Definition of cultural heritage: references to documents in history’, ICCROM Working Group ‘Heritage and Society’, (January), 4–8.
JOSEPH, E. (ed.) (2021) Microorganisms in the Deterioration and Preservation of Cultural Heritage. Cham: Springer International Publishing. https://doi.org/10.1007/978-3-030-69411-1. DOI: https://doi.org/10.1007/978-3-030-69411-1
MÁRCIA AIKO, J. S. et al. (2011) ‘Effect of culture medium on biocalcification by pseudomona putida, lysinibacillus sphaericus and bacillus subtilis’, Brazilian Journal of Microbiology, 42(1517–8382). DOI: https://doi.org/10.1590/S1517-83822011000200014
LE MÉTAYER-LEVREL, G. et al. (1999). ‘Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony’, Sedimentary Geology, 126(1–4): 25–34. https://doi.org/10.1016/S0037-0738(99)00029-9. DOI: https://doi.org/10.1016/S0037-0738(99)00029-9
MICALLEF, R. et al. (2016). ‘Biocalcifying Bacillus subtilis cells effectively consolidate deteriorated Globigerina limestone’, Journal of Industrial Microbiology and Biotechnology, 43(7): 941–952. https://doi.org/10.1007/s10295-016-1768-0. DOI: https://doi.org/10.1007/s10295-016-1768-0
DE MUYNCK, W. et al. (2011). ‘Influence of pore structure on the effectiveness of a biogenic carbonate surface treatment for limestone conservation’, Applied and Environmental Microbiology, 77(19: 6808–6820. https://doi.org/10.1128/AEM.00219-11. DOI: https://doi.org/10.1128/AEM.00219-11
ORTEGA-MORALES, B. O. AND GAYLARDE, C. C. (2021). ‘Bioconservation of Historic Stone Buildings—An Updated Review’, Applied Sciences, 11(12): 5695. https://doi.org/10.3390/app11125695. DOI: https://doi.org/10.3390/app11125695
ORTEGA-VILLAMAGUA, E., GUDIÑO-GOMEZJURADO, M. AND PALMA-CANDO, A. (2020). ‘Microbiologically Induced Carbonate Precipitation in the Restoration and Conservation of Cultural Heritage Materials’, Molecules, 25(23): 3–6. https://doi.org/10.3390/app1112569510.3390/molecules25235499. DOI: https://doi.org/10.3390/molecules25235499
PERALTA, E. AND MOYA, R. (2007). Quito: Patrimonio Cultural de la Humanidad. Maxigraf S.A.
PÉREZ, H. F. AND GARCÍA, M. G. (2020). ‘Bioprecipitation of calcium carbonate by Bacillus subtilis and its potential to self-healing in cement-based materials’, Journal of Applied Research and Technology, 18(5). https://doi.org/10.22201/icat.24486736e.2020.18.5.1280. DOI: https://doi.org/10.22201/icat.24486736e.2020.18.5.1280
PIGGOT, P. J. (2009). ‘Bacillus Subtilis’, in Encyclopedia of Microbiology. 45–56. https://doi.org/10.1016/B978-012373944-5.00036-5. DOI: https://doi.org/10.1016/B978-012373944-5.00036-5
SLEPECKY, R. A. AND HEMPHILL, E. H. (2006). The Genus Bacillus-Nonmedical. https://doi.org/10.1007/0-387-30744-3. DOI: https://doi.org/10.1007/0-387-30744-3_16
SOFFRITTI et al. (2019). ‘The Potential Use of Microorganisms as Restorative Agents: An Update’, Sustainability, 11(14). https://doi.org/10.3390/su11143853. DOI: https://doi.org/10.3390/su11143853
STEINBERG, D. et al. (2016). ‘Bacillus subtilis manual’, Molecular Microbiology, 1012–1025. https://doi.org/10.1111/j.1365-2958.2008.06467.x. DOI: https://doi.org/10.1111/j.1365-2958.2008.06467.x
ZHENG, T. AND QIAN, C. (2020) ‘Influencing factors and formation mechanism of CaCO3 precipitation induced by microbial carbonic anhydrase’, Process Biochemistry, 91: 271–281. https://doi.org/10.1016/j.procbio.2019.12.018. DOI: https://doi.org/10.1016/j.procbio.2019.12.018
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